1997-387

Time- and dose-dependent biomarker responses in flounder (Platichthys flesus L) exposed to benzo[a]pyrene, 2,3,3',4,4',5-hexachlorobiphenyl (PCB-156) and cadmium

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Title:Time- and dose-dependent biomarker responses in flounder (Platichthys flesus L) exposed to benzo[a]pyrene, 2,3,3',4,4',5-hexachlorobiphenyl (PCB-156) and cadmium

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Articles (Tidsskriftartikler)
Authors:Beyer, J.
Sandvik. M.
Skare, J.U.
Egaas, E.
Hylland, K.
Waagbo, R.
Goksøyr, A.
Research group:Biomiljø (Biomiljø)
Distribution restriction:Open
Published by:BIOMARKERS,
;
Additional information:
Biomarkers, Vol. 2, 1997, pp 35-44;Published by: BIOMARKERS;

Abstract:
Responses in flounder (Platichthys flesus) towards benzo [a]pyrene (BaP), ;2,3,3',4,4',5-hexachlorobiphenyl (PCB-156), and cadmium (Cd) were investigated ;in time-course and dose-response studies of selected biomarkers. Measurements ;of biliary fluorescent BaP metabolites and hepatic concentrations of PCB-156 ;and cadmium showed that the injected toxicants were rapidly mobilized from the ;muscle to the liver, but a depot effect was indicated in the highest dose ;groups of BaP and PCB-156 (12 mg kg(-1) bodyweight). Clearest biomarker ;responses were found in the induction of hepatic cytochrome P450 1A (CYP1A) ;enzymes as a response towards BaP and PCB-156 exposure. Maximum induction of ;CYP1A dependent 7-ethoxyresorufin-O-deethylase (EROD) activity was observed ;after 2 and 8 days in BaP and PCB-156-treated flounder, respectively. Positive ;dose-effect relationships were observed towards both compounds, but the CYP1A ;induction was move persistent with PCB exposure than with BaP exposure. In ;Cd-exposed fish, the hepatic l;ever of metallothionein responded more slowly with highest levels observed ;after 16 days in the time-study, in the combined BaP Cd treatment, the CYP1A ;induction was only slightly suppressed. Aspartate aminotransferase in serum ;appeared to be responsive towards BaP, but also towards the acetone vehicle in ;controls in the first part of the exposure period. Hematocrit as well as ;hepatic activities of aldrin epoxidase, glutathione S-transferase, and ;UDP-glucuronyl transferase were not responsive to any treatment in the present ;study. in general, the results demonstrate that selected biomarkers in flounder ;are responsive to PAH, PCB, and heavy metal pollutant exposure, indicating the ;applicability of this species in future environmental pollution monitoring ;programmes.;

Keywords:
fish;biomarkers;pah;pcb;heavy metals;perch perca-fluviatilis;rainbow-trout;biotransformation enzymes;monooxygenase induction;pleuronectes-platessa;stenotomus-chrysops;cytochrome p4501a;different tissues;mono-ortho;liver;;;;;;;;;;;;

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International Research Institute of Stavanger
Mailing address:
Visiting address:
P.O. Box 8046, N-4068 Stavanger, Norway
Prof. Olav Hanssensvei 15, 4021 Stavanger


Phone:
Fax:

+47 51 87 50 00
+47 51 87 52 00

General email address: firmapost@iris.no