2004-279

Assessment of environmental pollution in the North Sea through the analysis of fish bile with DNA-based biosensor

Author: 

;;;;

;;;;;;;;;
Title:Assessment of environmental pollution in the North Sea through the analysis of fish bile with DNA-based biosensor

Category:

Conferences (Konferanser)
Authors:Bagni, Graziana
Hernandez, Silvia
Baussant, T.
Mascini, Marco
Research group:Biomiljø (Biomiljø)
Distribution restriction:Open
Order no.:2004/279
Price.:60
;
Additional information:
European Conference on Analytical Chemistry The Role of Analytical Chemistry in the protection of the Citizens, 5-10 September 2004 Salamanca (Spain);

Abstract:
DNA based biosensors represent a new research field with interesting ;possibilities for practical application in various fields such as environmental ;and medical screening. Many molecules show a high affinity for DNA and they can ;interact with the nucleic acids immobilised on the electrode surface. The ;interactions between DNA and environmental pollutants can cause chemical and ;conformational modifications of nucleic acids and thus variation of the ;electrochemical properties of DNA. The presence of these compounds is measured ;by their effect on the guanine base: the changes in oxidation of the guanine ;peak, obtained by a square wave voltammetric scan, is used as analytical signal ;[1].;Measurement of contaminants or their metabolites in tissues or body fluids of ;organisms were exploited in this research. A rapid and low cost device, based ;on electrochemical DNA biosensor, is proposed as a screening tool for the ;detection of PAH exposure at contaminated sites. Laboratory studies have ;demonstrated that the presence of PAH metabolites in bile is well correlated ;with levels of exposure [2]. The gallbladder bile is a major excretion route ;for PAH in fish. After biotransformation, PAH metabolites are excreted into the ;bile and concentrated. Thus, the bile can be used as an indicator of PAH ;exposure. ;Preliminary studies with PAH metabolites standard solution were performed, ;using naphthalene, phenantrene, pyrene and benzo[a]pyrene hydroxy or dihydroxy ;as model compounds. The effect of these compounds on the surface-confined DNA ;was found to be linearly related to the concentration of the PAH derivative in ;solution. Then, the DNA biosensor was used to investigate the genotoxic effect ;due to the presence of PAH metabolites in fish bile samples. Two different ;sample groups were analysed: bile analysis of fish injected with a single PAH ;and fish caught bile samples at different sampling sites in the North Sea. A ;one-way analysis of variance (ANOVA) was used to compare between the different ;sample groups. When the ANOVA indicated that significant differences existed, ;Fisher´s multiple comparison test was then used. Dunnett’s test was used to ;determine the significance of differences between the reference sample groups ;and the control (blank assay) group. A significance level of P<0.05 was="" applied="" in="" all="" statistical="" tests.="" the="" results="" demonstrated="" that="" the="" sampling="" sites="" presented="" different="" pollution="" levels="" and="" that="" the="" pah="" derivative="" presented="" different="" genotoxic="" effect.="" the="" results="" obtained="" with="" the="" biosensor="" analysis="" were="" compared="" with="" fixed="" wavelength="" fluorescence="" (ff)="" at="" the="" excitation/emission="" wavelenghts="" of="" pyrene="" (340/380nm)="" and="" benzo[a]pyrene="" (380/430nm)="" and="" a="" good="" correlation="" was="" found.="">

<< Back

International Research Institute of Stavanger
Mailing address:
Visiting address:
P.O. Box 8046, N-4068 Stavanger, Norway
Prof. Olav Hanssensvei 15, 4021 Stavanger


Phone:
Fax:

+47 51 87 50 00
+47 51 87 52 00

General email address: firmapost@iris.no
International Research Institute of Stavanger
Mailing address:
Visiting address:
P.O. Box 8046, N-4068 Stavanger, Norway
Prof. Olav Hanssensvei 15, 4021 Stavanger


Phone:
Fax:

+47 51 87 50 00
+47 51 87 52 00

General email address: firmapost@iris.no